We're excited to announce the launch of Harbor™, our proprietary targeted integration technology that dramatically accelerates stable cell line generation. This breakthrough platform reduces timeline from months to just three weeks while maintaining high efficiency and reproducibility.
The Challenge of Stable Cell Line Development
Traditional stable cell line generation faces several limitations:
- Time-consuming: Random integration approaches require 2-3 months of selection and screening
- Variable expression: Random genomic insertion leads to unpredictable expression levels
- Low efficiency: Multiple rounds of screening needed to identify suitable clones
- Silencing risk: Transgenes may be silenced over time due to epigenetic effects
Harbor™ Technology: A Game-Changer
Our Harbor™ system combines:
Proprietary Harbor Cell Lines: Pre-engineered with optimized genomic safe harbor sites that ensure:
- Consistent, high-level transgene expression
- Stable expression over extended passages
- Minimal impact on endogenous gene function
- Compatibility with multiple cell types (HEK293, CHO, Jurkat, and more)
pCargo Vector System: Streamlined cloning vectors featuring:
- Flexible multiple cloning sites for easy gene insertion
- Selection markers for efficient clone isolation
- Optimized regulatory elements for robust expression
- Compatibility with standard molecular biology workflows
Validated Integration Protocol: Optimized procedures ensuring:
-
80% integration efficiency
- Single-copy integration for most applications
- Rapid selection (7-10 days)
- Complete characterization and validation
Key Applications
Harbor™ technology is ideal for:
- Recombinant protein production: Generate stable producer cell lines for antibodies, enzymes, or therapeutic proteins
- Reporter cell lines: Create sensitive biosensors for drug screening or pathway analysis
- Overexpression studies: Stably express genes of interest for functional characterization
- CRISPR applications: Integrate Cas9 or other genome editing components for stable expression
Performance Metrics
In validation studies, Harbor™ demonstrated:
- 3-week turnaround from transfection to validated clone
- 85% average integration efficiency
- <5% variation in expression levels across clones
- Stable expression maintained for >50 passages
Pricing and Availability
Harbor™ products are now available for order:
- Harbor™ Cell Lines: Starting at $2,500
- pCargo Vectors: Starting at $500
- Custom Integration Services: Contact for quote
Special introductory pricing available for academic institutions and first-time customers.
Conclusion
Harbor™ technology represents a significant advancement in stable cell line generation, offering speed, efficiency, and reliability that traditional methods cannot match. Contact our team today to learn how Harbor™ can transform your cell line development workflow.